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osteonectin  (R&D Systems)


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    R&D Systems osteonectin
    Osteonectin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteonectin+sparc/Human+SPARC+Antibody/pm41315304-215-9-15
    Average 93 stars, based on 13 article reviews
    osteonectin - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Morphometric analysis of calcification and fibrous layer thickness in carotid endarterectomy tissues
    Article Snippet: .. Mouse anti-human monoclonal antibodies against α-smooth muscle actin (DAKO N-1584, Carpinteria, CA), CD 68 (DAKO N1577, Carpinteria, CA), osteopontin (R&D MAB-1433, Minneapolis, MN), osteocalcin (R&D MAB-1419, Minneapolis, MN) and osteonectin/SPARC (R&D MAB-941, Minneapolis, MN) were applied to the tissue sections at concentrations recommended by the suppliers for immunohistochemistry identification ( Supplemental Figure 1 ). .. Biotinylated antibodies (Vector Laboratories, Burlingame, CA) against mouse IgG were applied as secondary antibodies.

    Immunohistochemistry:

    Article Title: Morphometric analysis of calcification and fibrous layer thickness in carotid endarterectomy tissues
    Article Snippet: .. Mouse anti-human monoclonal antibodies against α-smooth muscle actin (DAKO N-1584, Carpinteria, CA), CD 68 (DAKO N1577, Carpinteria, CA), osteopontin (R&D MAB-1433, Minneapolis, MN), osteocalcin (R&D MAB-1419, Minneapolis, MN) and osteonectin/SPARC (R&D MAB-941, Minneapolis, MN) were applied to the tissue sections at concentrations recommended by the suppliers for immunohistochemistry identification ( Supplemental Figure 1 ). .. Biotinylated antibodies (Vector Laboratories, Burlingame, CA) against mouse IgG were applied as secondary antibodies.

    Immunohistochemical staining:

    Article Title: Differential proteome profile, biological pathways, and network relationships of osteogenic proteins in calcified human aortic valves.
    Article Snippet: Calcific aortic valve disease (CAVD) is the most common heart valve disease requiring intervention.. Most research on CAVD has focused on inflammation, ossification, and cellular phenotype transformation.. To gain a broader picture into the wide range of cellular and molecular mechanisms involved in this disease, we compared the total protein profiles between calcified and non-calcified areas from 5 human valves resected during surgery.

    Article Title: Differential proteome profile, biological pathways, and network relationships of osteogenic proteins in calcified human aortic valves
    Article Snippet: .. Following an antigen retrieval step (0.01 M citric acid), sections were processed through standard immunohistochemical procedures to detect osteopontin (MAB1433, R&D Systems), osteonectin/SPARC (MAB941, R&D Systems), osteocalcin (MAB1419, R&D Systems), osteoprotegerin (MA5-15960, Thermo Scientific), and RANK/CD265 (9A725, Thermo Scientific). ..



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    Image Search Results


    MSCs differentiation in cultures over artificial hFGF2-H6 SGs PEA–FN surfaces. (A) Imaging of MSCs by fluorescence microscopy in the presence of artificial hFGF2-H6 SGs at 50 ng/mL for 14 days. Actin, vinculin, vimentin, and YAP were selected as cell markers. Merge refers to actin and vinculin combined fluorescence signals. White squares highlight cell focal adhesions. Close-up pictures of focal adhesions are displayed on the right panels. (B) Fold change on mRNA content (meaning RUNX2, OSN; osteonectin, and OPN; osteopontin gene expression) in MSCs upon incubation with soluble hFGF2-H6 (dark blue), artificial hFGF2-H6 SGs (pale blue), and free Zn 2+ (gray) at 50 ng/mL for 14 days. (C) In-cell Western (ICW) immunodetection of RUNX2, OSN, and OSP proteins in MSCs extracts upon incubation over soluble hFGF2-H6, artificial hFGF2-H6 SGs, and free Zn 2+ (gray) at 50 ng/mL for 14 days. The protein signal is displayed in green, and the cell signal is in red. (D) Statistical analysis of protein signal (green from panel C) expressed as fluorescence per cell and cm 2 in absorbance units). (E) Statistical analysis of cell signal (red from panel C) expressed as fluorescence per cm 2 in absorbance units (au). Peak numbers correspond to the increased percentage of cell growth comparing artificial hFGF2-H6 SGs (pale blue) with soluble hFGF2-H6 (dark blue), free Zn 2+ (gray), and control MSCs (black). Data are expressed as mean ± SEM, and statistical significance is achieved when p < 0.05 is represented as (*). Control refers to MSCs seeded on top of FN-PEA surfaces.

    Journal: ACS Applied Materials & Interfaces

    Article Title: Hybrid Micro-/Nanoprotein Platform Provides Endocrine-like and Extracellular Matrix-like Cell Delivery of Growth Factors

    doi: 10.1021/acsami.4c01210

    Figure Lengend Snippet: MSCs differentiation in cultures over artificial hFGF2-H6 SGs PEA–FN surfaces. (A) Imaging of MSCs by fluorescence microscopy in the presence of artificial hFGF2-H6 SGs at 50 ng/mL for 14 days. Actin, vinculin, vimentin, and YAP were selected as cell markers. Merge refers to actin and vinculin combined fluorescence signals. White squares highlight cell focal adhesions. Close-up pictures of focal adhesions are displayed on the right panels. (B) Fold change on mRNA content (meaning RUNX2, OSN; osteonectin, and OPN; osteopontin gene expression) in MSCs upon incubation with soluble hFGF2-H6 (dark blue), artificial hFGF2-H6 SGs (pale blue), and free Zn 2+ (gray) at 50 ng/mL for 14 days. (C) In-cell Western (ICW) immunodetection of RUNX2, OSN, and OSP proteins in MSCs extracts upon incubation over soluble hFGF2-H6, artificial hFGF2-H6 SGs, and free Zn 2+ (gray) at 50 ng/mL for 14 days. The protein signal is displayed in green, and the cell signal is in red. (D) Statistical analysis of protein signal (green from panel C) expressed as fluorescence per cell and cm 2 in absorbance units). (E) Statistical analysis of cell signal (red from panel C) expressed as fluorescence per cm 2 in absorbance units (au). Peak numbers correspond to the increased percentage of cell growth comparing artificial hFGF2-H6 SGs (pale blue) with soluble hFGF2-H6 (dark blue), free Zn 2+ (gray), and control MSCs (black). Data are expressed as mean ± SEM, and statistical significance is achieved when p < 0.05 is represented as (*). Control refers to MSCs seeded on top of FN-PEA surfaces.

    Article Snippet: Cells were incubated with monoclonal primary Abs (1:200) in blocking buffer (PBS/1% milk protein) at room temperature for 2.5 h, respectively; Runx2 (Santa Cruz Biotechnology, C1319), osteonectin (Santa Cruz Biotechnology, SC398419), and osteopontin (Santa Cruz Biotechnology, B1218).

    Techniques: Imaging, Fluorescence, Microscopy, Gene Expression, Incubation, In-Cell ELISA, Immunodetection, Control